Coding

Part:BBa_M36006:Design

Designed by: Jesse Palmer, Elliot Lui   Group: Stanford BIOE44 - S11   (2011-04-26)

glucose1phosphate adenylyltransferase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 571
    Illegal EcoRI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 571
    Illegal EcoRI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 571
    Illegal EcoRI site found at 1068
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 571
    Illegal EcoRI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 571
    Illegal EcoRI site found at 1068
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This mutation results in greater glycogen production compared to WT.


Source

The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation of the glgC gene.

References

Meyer CR, Ghosh P, Remy E, Preiss J. Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B. J Bacteriol. 1992 Jul;174(13):4509–4512

http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=GLYCOGENSYNTH-PWY&detail-level=3